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Introduction
This program was developed
to help in the semiquantitave evaluation
of immunohistochemical staining. We
attempted to create some easy to use and
interactive tool to classify and count
the nuclear staining (steroid receptors,
Ki67, p53 etc.) in tumours. A function
"Histoscore" is implemented.
The goal was to automate the
classification and counting of stained
nuclei, while their selection remained
interactive.
ColGraphAn is based on the evaluation of
colour within each "target"
(i.e. circle of adjustable size). The
localisation of targets is user defined
(i.e. they can be put over the objects of
interest e.g. tumour nuclei). These
targets are then sorted (different
sorting algorithms available) and
classified according to the user defined
"etalon" into 2-6 user-defined
categories. The construction of etalons
ensures they can be updated for
previously non-recognised colour, but
they still can remain backward
compatible.
The program loads previously captured and
saved (CCD camera) colour images. The
counting results can be easily saved
either as an image with classified
targets and/or as a tabular output which
can be transferred into any spreadsheet
e.g. MS Excel.
The results sort all methods you can see
in HTML code. The results calculation can
be exported as Bitmap, JPEG or GIF images
in order to process them in another
application.
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